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Nycomed ficoll histopaque 1077 lymphoprep
Ficoll Histopaque 1077 Lymphoprep, supplied by Nycomed, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphoprep+1077/ficoll+histopaque+1077+lymphoprep/pm25619642-58-38-42
Average 90 stars, based on 1 article reviews
ficoll histopaque 1077 lymphoprep - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Cell Culture:

Article Title: Identification, immunomodulatory activity, and immunogenicity of the major helper T-cell epitope on the K blood group antigen.
Article Snippet: PBMCs were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: Isolation and culture of peripheral blood mononuclear cells PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: 30 – 32 Cell culture As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.

Isolation:

Article Title: Identification, immunomodulatory activity, and immunogenicity of the major helper T-cell epitope on the K blood group antigen.
Article Snippet: PBMCs were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: Isolation and culture of peripheral blood mononuclear cells PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: 30 – 32 Cell culture As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.

Gradient Centrifugation:

Article Title: Identification, immunomodulatory activity, and immunogenicity of the major helper T-cell epitope on the K blood group antigen.
Article Snippet: PBMCs were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: Isolation and culture of peripheral blood mononuclear cells PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: 30 – 32 Cell culture As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.

Modification:

Article Title: Identification, immunomodulatory activity, and immunogenicity of the major helper T-cell epitope on the K blood group antigen.
Article Snippet: PBMCs were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: Isolation and culture of peripheral blood mononuclear cells PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Production of the effector cytokine interleukin-17, rather than interferon-?, is more strongly associated with autoimmune hemolytic anemia
Article Snippet: PBMC were isolated from fresh whole blood by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark).

Article Title: Combination peptide immunotherapy suppresses antibody and helper T-cell responses to the RhD protein in HLA-transgenic mice
Article Snippet: 30 – 32 Cell culture As previously described, 1.25×10 6 human PBMC/mL, isolated by density gradient centrifugation (Lymphoprep 1077; Nycomed Denmark) 1 , 28 or 2×10 6 murine splenic mononuclear cells (SMC)/mL 2 were cultured in alpha modification of Eagles medium (Gibco/Invitrogen, Paisley, UK) supplemented with 1% 2 mM L-glutamine (Invitrogen), 2% 20 mM HEPES buffer (Sigma, Poole, UK) and 2% penicillin streptomycin (Invitrogen), at 37°C in a humidified atmosphere of 5% CO2/95% air.



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